Overview of 49 CFR Part 40
The Department of Transportation’s (DOT) rule, 49 CFR Part 40, describes required procedures for conducting workplace drug and alcohol testing for the Federally regulated transportation industry.
To view 49 CFR Part 40 by section and related Q&As, click on the relevant subpart below.
Urine Collection Personnel
Collection Sites, Forms, Equipment and Supplies Used in DOT Urine Collections
Urine Specimen Collections
Drug Testing Laboratories
Medical Review Officers and the Verification Process
Split Specimen Tests
Problems in Drug Tests
Alcohol Testing Personnel
Testing Sites, Forms, Equipment and Supplies Used in Alcohol Testing
Alcohol Screening Tests
Alcohol Confirmation Tests
|What are the first steps in an alcohol confirmation test?
|What are the procedures for conducting an alcohol confirmation test?
|What happens next after the alcohol confirmation test result?
Problems in Alcohol Testing
Substance Abuse Professionals and the Return-to-Duty Process
Confidentiality and Release of Information
Roles and Responsibilities of Service Agents
Public Interest Exclusions
|Appendix A to Part 40
|DOT Standards for Urine Collection Kits
|Appendix B to Part 40
|DOT Drug Testing Semi-Annual Laboratory Report to Employer
|Appendix C to Part 40
|DOT Drug Testing Semi-Annual Laboratory Report to DOT
|Appendix D to Part 40
|Report Format: Split Specimen Failure to Reconfirm
|Appendix E to Part 40
|SAP Equivalency Requirements for Certification Organizations
|Appendix F to Part 40
|Drug and Alcohol Testing Information that C/TPAs May Transmit to Employers
|Appendix G to Part 40
|Alcohol Testing Form (Required for use beginning 01/01/2011. Authorized for use effective 2/25/2010.)
|Appendix G Questions and Answers
|Appendix H to Part 40
|MIS Form and Instructions (Required for use beginning in 2011 to report calendar year 2010 MIS data.)